Cuvette matching refers to the degree to which absorbing cuvette give similar absorption or transmission readings when empty or full of water. This practice started early in the history of cell manufacturing for spectrophotometers, and the absorption instrument was a single beam (lack of the ability to automatically adjust to a blank). As the instrument improved the concept of matching, it became less important. Bad cuvette may appear to match, because measuring cells without samples will not test the accuracy of the path length value and the quality of the window size. The important elements of cuvette quality are listed below.
Material | Code | Usable Range | Deviations in matching |
Optical glass | G | 320~2500nm | 350nmMAX0.5% |
ES quartz glass | Q | 190~2500nm | 200nmMAX0.5% |
IR quartz glass | I | 220~3500nm | 2730nmMAX0.5% |
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